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KMID : 0613820140240010014
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Journal of Life Science
2014 Volume.24 No. 1 p.14 ~ p.19
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PtdIns(3,5)P2 5-phosphatase Fig4 Interacts with Kinesin Superfamily 5A (KIF5A)
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Jang Won-Hee
Seog Dae-Hyun
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Abstract
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Kinesin-1 consists of two heavy chains (KHCs), also called KIF5s, and two light chains (KLCs) that form a heterotetrameric complex. Here, we demonstrate the binding of a neuronal KHC, KIF5A, to the carboxyl (C)-terminal tail region of Fig4 (also known as Sac3), a phosphatase that removes the 5-phosphate from phosphatidylinositol-3,5-bisphosphate (PtdIns(3,5)P₂). Fig4 bound to the C-terminal region of KIF5A but not to other KHCs (KIF5B and KIF5C) and KLC1 in yeast two-hybrid assays. The interaction was further confirmed in a glutathione S-transferase pull-down assay and by co-immunoprecipitation. Anti-KIF5A antibody co-immunoprecipitated Fig4 with KIF5A from mouse brain extracts. These results suggest that kinesin-1 could transport the Fig4-associated protein complex or cargo in cells.
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KEYWORD
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Adaptor protein, Fig4, protein-protein interaction, kinesin-1, microtubule motors, Sac3
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